Importantly, we assessed the time course of the effect on NLGN1 by performing quantifications also in patients with amnesic mild cognitive impairment (aMCI), in triple transgenic (3xTg-AD) mice of 4, 12 and 18 months, and in mice submitted to 2, 4 and 6 days of Aβo1-42 injection in the hippocampus. The gene discussed is NLGN1; the disease is Alzheimer disease.