On the contrary, significant deviations in the total ASVs reads were observed in the prostate hyperplasia pool (365 903 normalized reads; 36.6%; 17 detected ASVs) and the EEC pool (only 115049 of all normalized reads belonged to ASVs; 11.5%; 21 selected ASVs) which suggests that the splicing pattern of HNF1B could be disease-specific according to the quality (paired colorectal pools) or quantity (EEC and prostate hyperplasia pools) in the analysed tissues. This evidence concerns the gene HNF1B and benign prostatic hyperplasia.