We selected and cloned the following FOXL2 mutants found in BPES patients based on the human FOXL2 mutation database40: Q53X, I80T, I84S, F167X, and W204X, found in type I BPES patients showing both POI and eyelid malformation, and N105S and N109K, found in type II BPES patients exhibiting eyelid malformation, but not POI (Fig. 7a). Here, FOXL2 is linked to Eyelid malformation.