We manipulated FADS1 expression in TU212 cells by lentivirus transfection to observe FADS1 bioactivity, after assessing FADS1 levels in laryngeal carcinoma cell lines (AMC-HN-8, TU212, TU686) (Supplementary Fig. 2A–C). The gene discussed is FADS1; the disease is laryngeal carcinoma.