Indeed, while miR-126-3p inhibition and miR-138 up-regulation resulted in the up-regulation of single myogenic genes (Figure 6D and Supplementary material online, Figure S4D), miR-335-inhibitor treated myxoma-derived CSCs show a coherent and significant up-regulation of the main cardiac transcription factors (GATA-4, MEF2C and NKX2.5,) ensuing to an up-regulation of the main contractile genes (MYH6, MYH7, TNNT2, and ACTC1) in vitro upon 14 days in myogenic differentiation media (Figure 6D, E ). The gene discussed is TNNT2; the disease is myxoma.