CD34 and acute myeloid leukemia: Single-cell RNA sequencing and tSNE-based clustering identified AEL cell populations expressing human MB, erythroblast, and AML leukemic stem cell (LSC) gene signatures (Figure 4E), and showed that the LSC-like population was identified by the same markers as the expanded CD71–CD235a–CD33+KIT+CD34+ progenitor subset, whereas cells expressing the MB and erythroblast signatures expressed CD33, and TFRC and GYPA (which encode CD71 and CD235a), respectively, consistent with the flow cytometry data (Figures S8C and S8D; KIT expression not detected in 10× data).