To explore the role of PXN‐AS1 in GBM progression, we reduced PXN‐AS1 expression in U251 and U87 cells by transfecting two specific PXN‐AS1 shRNAs (sh‐PXN‐AS1#1, sh‐PXN‐AS1#2). The gene discussed is PXN; the disease is glioblastoma.