FN1 and neoplasm: Wessely et al. (2019) also tested the use of the Boyden chamber to evaluate and compare the invasive activity of spheroids containing only tumor cells and spheroids containing a mixture of tumor and stem cells. Another study examined the adhesion and cytoskeletal migration of HT1080 fibrosarcoma cells and LX2 line stellate cells in a three-dimensional system using fibronectin, Matrigel and type I collagen as chemoattractants (Tovari et al., 2014).