GLUD1 and hepatocellular carcinoma: A systematic kinetic analysis of GDH1 activity in hepatoma mitochondria was undertaken to elucidate its kinetic parameters, including Km values for NH4+, 2-OG, and NADPH; catalytic efficiencies (Vmax/Km); Ka values for ADP and leucine; Ki value for GTP; Hill coefficient n; and allosteric transition constant L. For comparison, liver mitochondria GDH1 was also characterized.