Liu's group conducted a study combining HIFU with R837 or a TLR‐4 agonist, monophosphoryl lipid A (MPLA) encapsulated in PLGA nanoparticles, and an anti—CTLA‐4 antibody.[80] The lack of CD4+ and CD8+ T‐cell infiltration and the presence of abundant CD45+CD11b+Gr‐1+ MDSCs were observed in the distant tumor after treatment of primary tumors by thermal ablation alone. This evidence concerns the gene CD4 and neoplasm.