In our work, we found the Notch1 pathway was positively associated with ID4 expression both in MDA-MB-231 and MCF-7/Adr cells, and ID4 could activate CBF1 by directly binding to the CBF1 promoter region via combined with MyoD1, which was reported to be a negative transcriptor in breast cancer 35. The gene discussed is RBPJ; the disease is breast cancer.