QPCR assays indicated that CLIC2 mRNA was not significantly changed (p>0.05) between ESCC and NT groups (H vs H-NT, L vs L-NT and H-L vs H-L-NT) whereas CLIC3 and CLIC4 mRNA were down-regulated (p<0.05) and upregulated (p<0.05) in ESCCs, respectively (Figures 6A-C). The gene discussed is CLIC4; the disease is esophageal squamous cell carcinoma.