EGF and posterior cortical atrophy: To investigate whether CMTM5-v1 suppressed the proliferation properties of PCa cells under the induction of EGF, PC3 and DU145 cells were transiently transfected with CMTM5-v1 plasmids and were maintained in culture media supplemented with 1% FBS in the presence or absence of 20 ng/ml EGF.