Through in silico analyses of The Cancer Genome Atlas sarcoma specimens, it was shown that the presence of an oncogenic mutation and a heterozygous deletion in the remaining PTEN allele led to a lower expression of CD8+ T cell markers (granzyme A [GZMA], perforin 1 [PRF1], and cluster of differentiation 8A [CD8A]) and PD1 and interferon γ (IFNG) genes.107 This observation indicates that complete inactivation of PTEN is associated with a reduced CD8+ T cell density and activation within the sarcoma TME. This evidence concerns the gene PRF1 and sarcoma.