LGR5 and neoplasm: Also when LGR5–GFP+ and LGR5–GFP− cells were isolated from tumor organoids and used for organoid re-initiation, while subsequently being treated with 5-FU, the resulting cultures were dominated by LGR5–GFP+-expressing cells, independent from whether LGR5–GFP+ or LGR5–GFP− were used as starting material (Fig. 5g).