Therefore, in the present study, we explored the probable role(s) and underlying molecular mechanisms of MALAT1 in HCC-SCs by statistical analyses of HCC-related big data and the functional probe of the effect of short-hairpin RNA (shRNA)-mediated downregulation of MALAT1 expression using the human HCC cell lines, Huh7, Mahlavu, SK-Hep1, and hepatoblastoma cells HepG2, as well as HCC tumor xenograft mice models. This evidence concerns the gene MALAT1 and neoplasm.