In another study, melatonin treatment effectively downregulated TNF-α and IL-1β production in human RA synovial fibroblasts (the MH7A cell line) by suppressing PI3K/AKT, ERK, and NF-κB signaling and upregulating miR-3150a-3p expression [20]. The gene discussed is TNF; the disease is rheumatoid arthritis.