In this study, although the change did not reach a significant level, the ACh levels were lower in the brain of the lpar3 KO zebrafish group as compared to that of the control group, which can be associated with anxiety-like behavior, as shown by the abnormal exploratory behavior and hyperactivity-like behavior displayed in the behavior tests, and the loss of memory observed in the mutant fish. Here, LPAR3 is linked to Anxiety.