MAPK8 and metabolic dysfunction-associated steatotic liver disease: In addition, Western blot analysis was adopted to determine the expressions of MAPK‐related markers in liver tissues of NAFLD mice, and the results demonstrated that phosphorylated ERK and phosphorylated p38 expression potently reduced in liver tissues of mice injected with sh‐GRHL2, while no obvious change was seen in ERK, p38, JNK and phosphorylated JNK expression (Figure 5D).