In order to further explore the anti-thrombotic activity of components in DS–SQ herbal pair, DSS, PRAC, PRAL, MA, LA, SAB, SAA of DS and GRg1, GRb1 of SQ were selected, and PHZ-induced zebrafish thrombosis model was used to evaluated their anti-thrombotic activity. This evidence concerns the gene SH3BP5 and Venous thrombosis.