In this study, in order to determine Tks5 interactome in situ and therefore invadopodia molecular composition, we engineered MDA-MB-231 breast cancer cell lines expressing either Tks5α, or Tks5α lacking the PX domain fused to the biotin ligase BirA* and verified that this allowed the biotinylation and identification of invadopodia components. Here, SH3PXD2A is linked to breast carcinoma.