In the current study, we developed an in vitro co-culture model that accurately recapitulates CAR T-related CRS, in which activated CAR T cells released IFN-γ, activating monocytes and cytokine release such as TNF-α, MIP-1α, M-CSF, IL-6, MCP-1, IL-1β, and IL-8. Here, CCL3 is linked to congenital rubella syndrome.