Considering the results of the experiment with BMP4 antibody blocked by the immunizing human BMP4 peptide and the lack of both bands (~60 and 58 kDa) in case of heart lysate (Figure 2b) we have additionally analyzed the impact of disease (heart failure; HF) and voluntary wheel running (training) on the sum of upper and lower bands of BMP4 in the heart. This evidence concerns the gene BMP4 and heart failure.