In vivo experiments assessing subcutaneous tumor formation in mice showed that both tumor volume and tumor weight were greater in group A than in group B. In the early period when LV-GALC fibroblasts possess senescent properties (p < 0.05), P16, P21, and P53 were more highly expressed in group A mice (LV-GALC fibroblasts and LoVo cells) than group B mice (LV-NC fibroblasts and LoVo cells). Here, TP53 is linked to neoplasm.