Duplex PCR assays for the detection of co-infection with A. marginale (targeting the msp4 gene) and A. phagocytophilum (targeting the msp2 gene) in cattle (M’ghirbi et al., 2016) and duplex real-time PCR assays for the detection of co-infection with A. marginale (targeting the msp1β gene) and Anaplasma centrale (targeting the groEL gene) in sheep (Decaro et al., 2008) were previously reported. This evidence concerns the gene HSPD1 and coinfection.