We have previously shown that tumour-immune cell interaction through PD-L1/PD1 bidirectionally stimulated FKBP5 alternative splicing.7,17 We have also shown that expression of FKBP51s in tumour-infiltrating lymphocytes of melanoma tissues is influenced by tumour PD-L1 expression.7 Herein, using organoids generated by two different melanoma tissue samples and autologous PBMC co-cultures, we provide further evidence of the causative effect of PD-L1/PD1 interaction on FKBP5 alternative splicing. This evidence concerns the gene FKBP5 and neoplasm.