The predicted target transcripts of miR-638, miR-1388, novel miRNA 5_23875, and miR-16-5p belong to the PCP, Wnt/Ca+, and Wnt/β-catenin signaling pathways including the genes ATF2, LGR4, PRICKLE1, ROCK1, RSPO3, WNT5A, NFAT5, and PLCB1. Other pathways identified were related to osteoarthritis, RAN signaling, estrogen receptor signaling, D-myo-inositol-5-phosphate metabolism, or pyridoxal 5’-phosphate salvage pathway. The gene discussed is RSPO3; the disease is osteoarthritis.