The aims of this study are to induce PD-1 gene-disrupted EvCAR-T cells from human peripheral blood T cells with the help of a CRISPR/Cas9-based genome editing tool and to evaluate the in vitro antitumor effects of PD-1-disrupted EvCAR-T cells on human GBM cells in real time. Here, PDCD1 is linked to glioblastoma.