TNF and infection: To analyze the release of the cytokines TNF- α, IL-6, and IL-10, along with VEGF, during infection with ZIKV, we performed ELISAs on the supernatant of mast cells activated with 30 min of contact with the virus as well as the extracellular levels produced by 1 h of virus presence and an incubation of 6 or 24 h.