To investigate possible relations between the miRNAs differentially expressed in CRC cells in an environment-dependent manner and their target genes, we evaluated the expression changes of genes associated with cell–ECM interactions and gap junction categories including integrins ITGA2, ITGA4, ITGA5, ITGA6, ITGA8, ITGAV, ITGB6, and ITGB8 and platelet-derived growth factors PDGFA, PDGFB, PDGFC as well as its receptors PDGFRA and PDGFRB in CRC cells grown in MCS vs 2D. This evidence concerns the gene PDGFC and colorectal carcinoma.