Firstly, RT‐qPCR was conducted to examine the effects of E2F4 on the expression of C16orf74 in cervical cancer cells, and the result showed a progressive elevation in the expression of C16orf74 in HAND2‐AS1‐overexpressed cervical cancer cells upon E2F4 silencing, while the same trend was observed in cells with C16orf74 and HAND2‐AS1 overexpression (Figure 4A; P < .05). The gene discussed is HAND2; the disease is cervical cancer.