Moreover, RT‐qPCR was conducted, and the enhanced expression of C16orf74 was detected in the tumours with HAND2‐AS1 overexpression and E2F4 silencing or C16orf74 overexpression (Figure 5D; P < .05), further supporting the critical role of C16orf74 expression in the cervical tumour growth. This evidence concerns the gene E2F4 and uterine cervix neoplasm.