ORAI1 and osteosarcoma: We used an osteosarcoma cell line with high metastatic potential, proven by a xenotransplant in zebrafish larvae, to analyze ORAI1 enrichment at the leading edge by means of immunofluorescence co-localization, and co-precipitation with cortactin, ARP2/3, and CYFIP1, which are at highly enriched levels in lamellipodia.