The siRNA-mediated loss of function study in HCC cells showed that transfection with two siRNAs (siSPR#1 and siSPR#2) resulted in more than 80% decrease in the SPR gene and protein expression in SMMC-7721 and BEL-7402 cells (Fig. 2a, b). This evidence concerns the gene SPR and hepatocellular carcinoma.