To determine whether there may be a role for SARM1 in mediating the destruction of NAD+/NADH pool in our rho−/− model of retinal degeneration, retinal explants were prepared from both rho−/−sarm1−/− or rho−/− mice at 4 wk of age, and NADH photon emission was assayed by FLIM (Fig 5C and D). This evidence concerns the gene RHO and retinal degeneration.