We previously demonstrated that patients afflicted with cryopyrin-associated periodic syndrome and juvenile idiopathic arthritis were classified separately from patients with FMF by the ex vivo colchicine assay.24 To further assess specificity of the assay, we evaluated the response of patient groups suffering from AIDs of which the pathophysiological mechanisms have been linked to deregulated activation of the Pyrin inflammasome. Here, NLRP3 is linked to familial Mediterranean fever.