To validate the function of HVEM in ovarian cancer under hypoxic microenvironment, OVCAR3 cells and primary ovarian cancer cells were cultured under hypoxic conditions, which mimicked a more realistic tumor environment, and the expression of HIF-1α and HVEM in the hypoxic ovarian cancer cells was detected by qRT-PCR and western blot. This evidence concerns the gene HIF1A and ovarian cancer.