A study done in MDA MB-231 breast cancer cells26 revealed that knocking down ZEB1 produced downregulation of more than 30 genes and upregulation of more than 200, many of which affected epithelial cell adhesion genes including the classic cadherin superfamily, components of tight junctions (e.g. occluding, claudin 7) desmosomes such as desmoplakin, plakohilin3 and desmocollin 2 and gap junctions such as connexin 26 and 31. This evidence concerns the gene ZEB1 and breast cancer.