Congruent with cell viability assays, DAZAP1 siRNA accelerated colony formation of KYSE450 and KYSE510 cells (all P<0.01) (Figure 3E), while the over-expression of DAZAP1 significantly suppressed colony formation of ESCC cells (both P<0.001) (Figure 3F). The gene discussed is DAZAP1; the disease is esophageal squamous cell carcinoma.