The inclusion of the alternative splicing exon (exon 26) was then examined in ESCC cells, either co-transfected with the TSC2-minigene construct, pcDNA3.1-DAZAP1 or different DAZAP1 siRNAs, respectively (Figure 5E and Figure S5). Here, DAZAP1 is linked to esophageal squamous cell carcinoma.