In order to assess the universality of the DNMT1/miR-34a/FoxM1 axis in LCSLCs, we selected two additional established liver cancer cell lines, including SK-Hep-1 and Hep G2 cells, to compare DNMT1 activities and protein amounts, miR-34a-5p levels, FoxM1 protein amounts, and stemness features between monolayer cells and the corresponding spheres, namely, LCSLCs. This evidence concerns the gene DNMT1 and liver cancer.