To verify the role of the AGEs‐RAGE/p38 MAPK/p65 NF‐κB signalling pathway in the protective effects of loganin on DM‐induced testicular damage, GC‐2 cells were pre‐treated with loganin (10 μmol/L) plus specific inhibitors, including RAGE blocking agent FPS‐ZM1, p38 MAPK inhibitor SB203580 or p65 NF‐κB inhibitor PDTC, for 1 hour. This evidence concerns the gene NFKB1 and diabetes mellitus.