Several known hypermutant tumour samples were deliberately run first, including a somatic POLE mutant colorectal cancer (reported TMB 261.71 mut/Mb in WGS sample), a somatic MLH1 mutant colorectal cancer (reported TMB 67.43 mut/Mb) and a somatic MSH6 mutant colorectal cancer (reported TMB 104.0 mut/Mb). The gene discussed is MLH1; the disease is colorectal cancer.