TSC2 and Anxiety: Reports that did not account for sex did not find any anxiety-like behavior in Tsc2 heterozygous mice (Ehninger et al., 2008, 2012; Potter et al., 2013; Tang et al., 2014), though Tsc2 dominant negative mice (balanced mix of both sexes) did show a trend toward anxiety-like behavior as measured in the open-field (Ehninger and Silva, 2011).