MYH14 and Duchenne muscular dystrophy: The 3 DMD samples had a combined average of 32% myofibre positivity for f/d myosin with DMD_2 having the highest value of 42% (Fig. 5a). Fig. 5b provides representative examples of f/d myosin immunostaining in CTRL_1, BMD_3 and DMD_3 along with their corresponding myosin classification maps generated via digital analysis.