In our study, there were three key observations: (1) MMP9 was upregulated by ZIKV infection both in vivo and in vitro; (2) MMP9 played an important role in promoting ZIKV entry into the testes by disrupting the BTB; and (3) ZIKV NS1 interacted with MMP9 and facilitated K63-linked polyubiquitination of MMP9. The gene discussed is MMP9; the disease is Zika virus infectious disease.