PH46 (2) reduced DAI in the acute 5% DSS and IL-10 -/- murine model of colitis and significantly reduced MPO activity, a measure of inflammatory cell infiltration and inflammatory colon cytokines (IL-6, IL-1β and TNF-α).[4] The observation of inhibition of NO and 5-LOX by PH46 (2) and other related indane molecules warrants further study and may help to elucidate a clearer understanding of the relationship between the reduction in inflammation in the in vivo models and the decreased level of cytokines observed. This evidence concerns the gene TNF and colitis.