Since metabolic reprogramming is a hallmark of cancer and differential metabolic reprogramming is important in the development and selection of effective therapies, we applied an exploratory, NMR-based metabonomics approach to characterize the metabolic pathways affected in isogenic SW48 cells harbouring different KRAS mutations at codons 12, 13, 61 and 146. The gene discussed is KRAS; the disease is cancer.