In the past, many non-tissue relevant and often cancerous cell lines have been used to express Cx26 mutants to uncover that GJB2 gene mutations linked to hearing loss fall into either loss-of-function or gain-of-function mutations (White, 2000; Zhao et al., 2006; Sanchez and Verselis, 2014). Here, GJB2 is linked to hearing loss disorder.