Mean fold change of TWIST1 expression in the Parsortix was 0.75 (range: 0–5.35) in HD, 0.10 (range: 0–2.04) in TWIST1 negative HNSCC patients and 227.19 (range: 18.90–982.29) in TWIST1 positive HNSCC patients. This evidence concerns the gene TWIST1 and head and neck squamous cell carcinoma.