Mean fold change of TWIST1 expression in the EpCAM-based enrichment was 0.92 (range: 0–3.16) in HD, 0.12 (range: 0–1.48) in TWIST1 negative HNSCC patients and 10.63 (range: 3.2–31.12) in TWIST1 positive HNSCC patients. Here, TWIST1 is linked to head and neck squamous cell carcinoma.