We also selected RASSF1A, since it has been shown that a high frequency of RASSF1A methylation is associated with more aggressive tumour phenotype among different cancer types9, while a recent meta-analysis suggested that there was a significant association between aberrant RASSF1A methylation and Head and Neck Squamous Cell carcinoma (HNSCC)10. Here, RASSF1 is linked to neoplasm.