We validated that miR-3619-5p interacted with HCP5 and could counteract the facilitative effect of HCP5 on stemness and chemo-resistance of GC cells, indicating that miR-3619-5p served as a chemo-sensitizer in GC cells and HCP5 regulated chemo-resistance of GC cells through miR-3619-5p. The gene discussed is HCP5; the disease is gastric cancer.