Interestingly, ISC-4 led to complete disappearance of p-Akt in the MV4-11 cells, instead, p-Akt in MOLM-13 and OCI-AML3 cells were still detectable at 24-h, represent residual p-Akt in AML (Figures 2A,B). This evidence concerns the gene AKT1 and acute myeloid leukemia.